Microwave and Vacuum Assisted Fixation and Cytochemical Localization for Lungs of Chicken, Gallus domesticus
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چکیده
Proper fixation of lung tissue for morphological and cytochemical studies by transmission electron microscopy (TEM) is difficult by conventional (perfusion and immersion) fixation processes. A broiler model (Gallus domesticus) of pulmonary hypertension was the source of lungs for this study. Microwave and intermittent vacuum were used in order to have better structural and enzymatic preservation. Bench cytochemical localization methods were modified for microwave use in terms of time and wattage. This paper presents the details of specimen preparation that were previously published to document oxidative stress by NADH oxidase (NOX) [1] in lungs of broilers maintained under hypoxia for four weeks.
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